VIVEK KUMAR AGNIHOTRI, PIYUSH KANT RAI AND KAMLESH CHOURE
Abstract
Water hyacinth (Eichhornia crassipes) is a pervasive aquatic weed that significantly impacts ecosystems and economies. This study aimed to identify fungal isolates with mycoherbicidal potential and develop effective formulations for controlling this invasive species. Genomic DNA from fungal isolates was extracted, and the 18srRNA region was amplified and sequenced. Phylogenetic analysis confirmed the identity of the isolates. The mycoherbicidal efficacy of these isolates was evaluated through in vivo and in vitro assays. Various liquid-based formulations were tested to optimize spore germination and seedling mortality, with Tween-80 emerging as the most effective adjuvant. Results demonstrated significant synergistic effects (up to 80%) when combining Alternaria alternata with other fungal pathogens, leading to enhanced disease progression on water hyacinth. The findings suggest that integrating multiple fungal species into a mycoherbicidal formulation could offer a promising strategy for managing water hyacinth infestations.