R.K. PATEL, S.M. OZAI, M.S. DODIA2 AND S.P. SINGH
Abstract
Three alkaliphilic actinomycetes were isolated from alkaline soil of Bhuj, Gujarat, India. All the three isolates were able to grow luxuriously at p1-I 10. Alkaline protease production was optimal in exponential phase of growth. The maximum enzyme secretion was observed in presence of Glucose (1%, w /v) and peptone (0.5%, w /v) as the sole source of carbon and nitrogen respectively. The growth and enzyme production was found to be suppressed with NI-11C1 (0.5%, w/v) and Urea (0.5%, w/v). Cations Ca, Mn++ (20 mM) repressed enzyme production significantly with higher growth. Whereas, Mg ++ (20 mM) found have lesser repressed effect on enzyme production with slight enhancement in growth. Whey supplemented with K2HPO4 (1%, w/v) enhanced the protease activity by two fold.