REEBA PANESAR, R.S. SINGH, P.S. PANESAR AND M.B. BERM
Abstract
The present investigation deals with the screening of different immobilization matrices to develop a suitable immobilized cell system for the lactose hydrolysis. The different immobilization matrices such as agarose, carrageenan, agar-agar alginate were used for immobilization of permeabilized yeast cells for their use in the lactose hydrolysis. The yeast cells immobilized in different matrices showed varied degree of hydrolysis. Out of these tested matrices, alginate entrapped cells showed maximum lactose hydrolysis. The maximum lactose hydrolysis was observed with 2% (w/v) alginate concentration after 150 min of incubation period.