T. VEERA NAGASUPRIYA, K. VENKATA LAXMI, P. SAIDAIAH, VEENA JOSHI AND M. GOPALA KRISHNA
Abstract
The present investigation was conducted to know the effect of different surface sterilization methods and different plant growth regulators on culture establishment from Pomegranate nodal explants of cv. Bhagwa with two factors: Sterilizing agents(S): 0.1% Mercuric chloride (HgCl2) and 1% Sodium hypochlorite (NaOCl) each at two different timing intervals i.e., 5 min and 10 min and growth regulators(G) G1: BAP 2 mg L-1, G2: Kinetin 2 mg L-1, G3: 2-ip 2 mg L-1, G4: zeatin 2 mg L-1). The results revealed that highest percentage of uncontamination was recorded in S2: 0.1% HgCl2 for 10min (93.33%) with lowest fungal contamination (5.83%) and bacterial contamination (0.83%) with no significant effect of growth regulators on contamination. Highest regeneration efficiency was recorded in BAP 2 mg L-1 (58.33%) with individual effect of sterilizing agent HgCl2 at 0.1% for 5 min in less number of days (9.53).