A. JAYAPRAKASH, A. MARY VIOLET CHRISTY, T.SHANKAR AND M. JAWAHAR
Abstract
All known enzyme are produced in living cells in deed, without enzymes living cells could not function. Enzymes are of many different types. They promote the synthesis and breakdown of biological molecules as well as a range of plant, microbes and animal tissues. Phosphatases are key enzymes in liberating and recycling the phosphate that is necessary for many fundamental biological processes. Hence in the present research programme planned to isolate the phosphate solubilizing bacteria from soil, to transform the bacterial plasmid to non-pathogenic fungi and evaluating the phosphate solubilizing capacity in transformed non pathogenic Fusarium. Genetic analysis of transformed non-pathogenic Fusarium species was analyzed in agarose electrophoresis along with bacillus subtilis chromosomal DNA and non-pathogenic Fusarium DNA With aid of transformation technology the compitant non-pathogenic Fusarium species has the ability to solubilize phosphate as well as it will act as a biocontrol agent.