S. RAJAN, S. BALAKUMAR AND T. THIRUNALASUNDARI
Abstract
Antibacterial activity of Punica granatum fruit rind extracts & fractions were studied on Enteropathogenic strains of Escherichia co/i. Disc diffusion method (Baiter et al- 1966) was adapted to screen antibacterial activity and agar dilution method (NCCLS , 1993) was used to look for Minimum Inhibitory Concentration (MIC). Antibacterial activity was observed in two extracts and various fractions. WC of the hexane & water fraction were found to be 50 ig / ml concentration and methanol, chloroform & benzene fractions were at 100 ig / ml. Aqueous extracts taken with ethyl alcohol and water showed MIC at 200 ig / ml concentrations.