B.S.PRAKASH KRISHNAN, A.MARY VIOLET CHRISTY AND SJAGAVEERA PANDIAN
Abstract
DNA finger printing is defined as the application of molecular marker techniques to identify cultivars. Plant DNA fingerprinting is intricate since it deals with populations and often more than one species. It require constant interaction with breeders, biometricians. computer analysis, legal experts and policy markers. Plant DNA finger printing is a powerful molecular technique to reveal differences in genomic DNAs. It has the advantage of following early detection, being constant in different growth conditions and throughout different developmental stages. The genetic diversity of mango available in India is very rich and at present more than one thousand vegetatively propagated varieties exists in the country. In the present study three commercially known varieties and three hybrid varieties (Ratanagiri,Totapuri,Samparpatti Totapuri) released by Indian Institute of Horticultural Research, were taken for developing DNA fingerprinting. The number of bands obtained for each of these varieties and their relative positions. RAPD and ISSR primers to generate to assess the genetic relatedness among mango varieties and hybrids.