P.K. CHOUHAN, P. DAS AND M. SHARMA
Abstract
Protein and amino acids are nitrogenous compounds and are also used as precursor molecule in protein synthesis. A lot of cellular and membrane components are made (UP) of protein; therefore organisms synthesized amino acids and protein during growing condition as primary metabolites. A cyanobacterium is most diverse photosynthetic nitrogen fixing group and their thallus vary from unicellular to filamentous. They found in terrestrial habitat and also in water and fix atmospheric nitrogen in both micro-aerophilic and aerobic condition, in aerobic condition nitrogen fix by a specialized cell found only in filamentous forms called heterocyst. This fixed nitrogen assimilated in form of amino acids and then proteins. Protein, amino acids, vitamins and plant growth hormones are liberate by cyanobacteria in culture media; therefore these nitrogenous compounds improve overall soil fertility. Our study based on estimation of total protein and identification of amino acids in culture filtrate, cyanobacterial strains were isolated from botanical garden soil of Govt. PG College, Mandsaur. This isolates were subjected to extracellular metabolite productions using BG-11 liquid medium with nitrogen, thereafter metabolites were estimated/identified using standard protocols.