DIRAYAH RAUF HUSAIN, FITRIANI, ZOHRA HASYIM, ASADI ABDULLAH AND SULFAHRI
Abstract
Earthworms are used as drugs, not only the compound earthworms but also endosymbiont microorganisms in earthworms suspected of having antibiotic activities. This research is aimed to identify the ability of several endosymbiont bacteria in Pheretima sp. earthworms to inhibit the growth of the bacteria Salmonella typhi and Staphylococcus aureus. Isolation of bacteria from Pheretima sp. earthworms was carried out on NA (nutrient agar medium. The observed characteristics of bacterial isolates from Pheretima sp.earthworms consisted of colony morphology, gram staining, and the presence of endospores. This research demonstrates the variety of colors, shapes, edges, and elevations of the isolates. The result showed that there are 2 isolate that was isolation form Pheretemia sp. namely Bacillus choshinensis and Bacillus brevis. Based on the test results of isolation and inhibition of Pheretima sp. earthworms, all isolate effectively inhibited the growth of the bacteria S. thypi and S. aureus, and they have the potential to act as antibiotics. Isolates Bacillus choshinensis had the largest inhibitory zone diameter of 21.32 mm against S. thypi after 15 days of incubation. Inhibitory properties of all isolates that were isolated from Pheretima sp. indicate that bacteria could be described as bactericidal.