IN VITRO PROPAGATION OF GRAPE ROOTSTOCK, DOGRIDGE AND 110 R: EFFECTS OF PGRS AND MEDIUM COMPOSITIONST. ANUPA, R.G. SOMKUWAR, A.K. SHARMA AND R.S. ROSHNIAbstract The objective of this studyâs is to develop a protocol for in vitro propagation of a grape rootstock. Dogridge and 110-R was chosen for their drought and salinity tolerance. In vitro culture can yield a large number of identical plantlets that can be preserved and used for a variety of purposes. In order to obtain high number of plants it is necessary to establish proper conditions of in vitro culture. Considering all the variables, the culture medium MS medium supplemented with 6 BAP @ 3.5 mg/l showed the best growth rates and development for shoots through nodal cutting and in vitro multiplication rate for dogridge and 110 R grape rootstock. Maximum rooting percentage and root growth was recorded with use of IBA+NAA (0.5+0.1 mg/l) in dogridge (68.3) and 110R (66.3). |