Ecology, Environment and Conservation Paper

ANALYSIS OF THE CONTENT OF SECONDARY METABOLITES USING UV-VIS AND FTIR SPECTROPHOTOMETRY FROM THE METHANOL EXTRACT OF RHIZOPHORA MUCRONATA LEAVES

Nelvan Subayu, Sri Andayani, Mohamad Fadjar and Ashari Fahrurrozi

Abstract

This study aims to determine the content of secondary metabolites in the methanol extract of R. mucronata mangrove leaves. The test was started from mangrove leaf extraction using methanol solution with a ratio of 1: 4 (b / v). Further analysis was performed using UV-VIS and FTIR spectrophotometry. The results of UV-VIS and FTIR are the absorbance values of a compound and its functional groups. The wavelengths generated from the UV-VIS analysis were 228, 236, 280 nm. These wavelengths are classified into flavonoids and tannins. While the results of long-wave FTIR are 3424.60, 2927.57, 1631.06, 1449.97. According to the results of the infrared spectrum in the presence of –OH, C-H (aromatic), C-O, C = C (aromatic) groups, it supports that the isolate is a compound of the phenolic group such as flavonoids and tannins. Flavonoids and tannins are secondary metabolite compounds that have anti-bacterial properties so that they can be used as medicinal agents, both for humans and animals.