Ecology, Environment and Conservation Paper

ASSESSMENT OF GENETIC DIVERSITY IN MANGO (MANGIFERAINDICA L.) BASED ON RAPD MARKERS

Upendra Maurya, Navneet Kumar, Arvind Kumar, Ujjwal Sirohi, Gaurav Kumar Ahirwar,Manoj Kumar Yadav, Lokesh Kumar Gangwar, Veena Chaudhary and Mukesh Kumar

Abstract

Assessment of genetic diversity in 24 Indian mango cultivars was carried out with eight Random AmplifiedPolymorphic DNA (RAPD) markers. A total of fifteen RAPD markers were screened out of which eightprimers produced polymorphism for finally selected for diversity analysis. Eight primers produced a totalof 20 bands with an average 2.5 bands per primer thus generated 100% polymorphism. Heterozygosityvaried from 0.278-0.49 while PIC was in ranged from 0.363 to 0.449. The highest Polymorphic Informationcontent (PIC) value (0.449) was observed for primer OPA 16 while lowest PIC value was observed in primerOPC 6 (0.363). Effective multiplex ratio was maximum (4.083) for the primer OPAB 11 and minimum effectivemultiplex ratio (0.163) showed by OPA 16 primer. Arithmetic means of H was maximum (0.014) for primerOPA 2 while minimum Arithmetic mean of H (0.003) exhibited by OPAB 11 primer. Maximum markerindex (0.012) exhibited by the primer OPA 18, OPA 11 and OPAB 10 while minimum marker index (0.002)observed with OPA 16 primer. Maximum discriminating power of marker (0.978) showed by OPA 16 andminimum discriminating power (0.397) observed with OPA 3 primer. Resolving power of markers variedin the range of 0.333 to 2.75. Maximum resolving power of markers (2.75) noted with OPC 6 while minimummarker resolving power (0.333) gave by OPA 16 primer. Jaccard’s similarity between pairs of cultivarsranged between 0.33 and 0.96. The cluster dendrogram of RAPD revealed 2 major clusters and cluster I wasthe small and included 3 genotypes while clusters II comprising 20 genotypes. Highest genetic similaritywas found between genotype Gulab Jamin and genotype Surkha, while the lowest genetic similarity wasobserved between genotype Manjeera and Alphonso and between Meetha Malda and Ramkela. Moleculardata produced by RAPD markers clearly indicates that genotypes like Malda Meetha, Rataul and BermaSurakh was found more divergent and can be used for further improvement programme.