Ecology, Environment and Conservation Paper

PRODUCTIVITY OF THE ENTOMOPATHOGENIC NEMATODE,HETERORHABDITIS INDICA ISOLATES IN GALLERIA MELLONELLA

Swati P. Shinde, V.K. Biradar, D.B. Ingole, N.V. Lavhe and Pragati R. Jawake

Abstract

Heterorhabditis indica, CICR-HI-MN and CICR-HI-CL isolates were tested over the fifth larval instar of theGreater wax moth, Galleria mellonella L. (Lepidoptera: Pyralidae), at nine different dosages, i.e., at 10, 20, 40,60, 80, 100, 120, 140, and 160 infective juveniles (IJs)/larva in 100 μl of sterile water under laboratory conditionat the district Nagpur of Vidharbha Region, India, during 2022. When the LC50 and LC95 values of 5thinstar larvae were analyzed, the CICR-HI-CL isolate was quite more efficient than CICR-HI-MN isolate.Moreover, when compared to various treatments, given isolates showed greater reproduction rate in thefifth instar, although the CICR-HI-MN isolate produced a higher emergence of infective juveniles with astatistically significant difference (P = 0.001). Native isolates of H. indica tested against fifth instar of Galleriamellonellalarvae exhibited excellent results and can be employed as the principal model for entomopathogenicnematodes maintenance and multiplication.