Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 04, Issue 4, 2002; Page No.(471-475)

PRODUCTION OF TRIGONOPSIS VARIABILIS IN CORN STEEP LIQUOR BASED FERMENTATION MEDIUM

PUNEET SINGHL, SATWINDER SINGH MARWAHA2 AND NEELAM VERMA

Abstract

The study was undertaken to conceive a corn steep liquor based fermentation medium of Trigonopsis variabilis for the production of D-amino acid oxidase. The fermentation profile of T. variabilis in the final medium showed that, a specific growth rate [u=0.34h-1], specific DAAO yfeld [Yxp=3004mg.g DW-1], biomass yield [X0=13.59gDW. I-1] and product yield [l'0=351U.GDW-1] were obtained during the fermentation at 30° C at 250rpm in 1.5 liter laboratory fermentor at pH 6.0 ± 0.05 after 40 hours.

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