MINING OF 1-AMINOCYCLOPROPANE-1-CARBOXYLIC ACID DEAMINASE EXHIBITING WATER STRESS TOLERANT NOVEL RHIZOBACTERIAL ISOLATESVIJAYKUMAR GANGARADDI, TAMILVENDAN K., MAHADEVSWAMY AND S.R. BALANAGOUDARAbstract An investigation was carried out to isolate, characterize and identify the rhizobacterial isolates of crop plants grown in the arid region of the North-eastern Karnataka. Twenty-one strains out of 120 rhizobacterial isolates that grew vigorously at-0.30Mpa were selected, and strains were biochemically characterized and screened in vitro to determine their water stress tolerance activity. Among 21 isolates, APPP-1 and GPPP-1 showed ACC deaminase activity (5.13 μMmg-1/h and 2.69μMmg-1/h, respectively). The isolate BSOP-1 produced higher proline (138.87μMmg-1) and IAA (68.49 μg ml-1); while NONB-2 produced higher exopolysaccharides (23.53mg mg-1 protein) at-0.30Mpa. Our study recorded the ACC Deaminase activity of the isolates, APPP-1and GPPP-1 later identified as a novel strain of Pseudomonas sp. VG309(NCBI accession number MH664930) and Corynebacterium kroppenstedtii (NCBI accession number MH671918) through 16SrDNA molecular sequencing technique. |