EXPRESSION PROFILE OF PROLACTIN GENE IN WHITE LEGHORN LAYER AND CONTROL BROILERB. RAJITH REDDY, B. EKAMBARAM, P. JAYA LAXMI, CH.HARIKRISHNA AND T.K. BHATTACHARYAAbstract Prolactin is an endocrine hormone involved in controlling broodiness in hens. Prolactin also inhibits egg production in chickens. The objective of the study is to see the prolactin expression profile using RT-qPCR during the 42nd week of egg production in the Indian white leghorn layer (IWI, IWK, and IWD) and control broiler. Five from each group are slaughtered and total RNA was isolated from the anteriorpituitary glands and cDNA was synthesized. The RT-qPCR studies revealed Prolactin concentration was highest in control broiler chicken compared to all other groups under study. The lowest Prolactin concentration was observed in the IWD layer compared to IWI and IWK layer strains. It is concluded that the prolactin gene of different chickens under experiment showed the presence of lower expression of the prolactin gene, which correlates with high egg production in layers. This experiment will form a base for further study of prolactin expression and chicken egg production in various breeds of poultry. |