STANDARDIZATION OF MICROPROPAGATION TECHNIQUE OF MULBERRY (MORUS ALBA L.)SWARNABH SWARNAM AND AFAQ MAJID WANIAbstract A standardization of protocol for rapid multiplication of mulberry (Morus alba L.) was established in vitro using nodal explant excised from hydroponically grown mature mulberry plants. Shoot initiation was induced on Murashige and Skoog (MS) medium supplemented with different concentrations of (BAP) 6- Benzyl amino purine. Effect of different concentration of 6- Benzyl amino purine was studied on multiplication of shoot, revealed that elongated shoots (2-3 cm) were cultured on half strength MS medium supplemented with (IBA) indole-3-butyric acid. Higher percentage of shoot initiation (90%) was observed on MS medium supplemented with 2 mg/l 6-bezyl amino purine. Initiated shoots gave a higher average number of shoots and elongation was observed when it was subcultured on MS medium supplemented with 1.4 mg/l 6-bezyl amino purine. A maximum percentage of root formation (70%) was observed when elongated shoots were subcultured on half strength MS medium containing 1.3 mg/l indole-3-butyric acid. Rooted plants transferred to pots containing sterile FYM, garden soil and sand (1:1:1) for acclimatization in green house showed (75%) survival capacity. |