EFFECTIVENESS OF ISSRS TO DETERMINE THE GENETIC RELATIONSHIPS BETWEEN THIRTY SPECIES FROM ELEVEN GENERA OF BAMBOORAHUL A. BAHULIKAR, SAGAR S. JADE AND HEMANT BEDEKARAbstract The identification of bamboo is traditionally dependent on vegetative characters due to its long and unpredictable flowering cycle. Molecular techniques are suited and consistent for resolving the genetic relationship between bamboo species and that was studied among 30 species from 11 genera using ISSRs markers. Thirteen ISSR primers revealed 305 polymorphic loci among the accessions Polymorphic information content and ISSR primer index ranged from 0.26 to 0.40 and 4.77 to 9.91 respectively. The Neighbour-Joining dendrogram of 30 species resulted in two major groups. all species of Bambusa were grouped in cluster I along with three species of Dendrocalamus, the remaining species of Dendrocalamus were distributed in cluster II along with nine other bamboo genera. This study supports the monophyletic origin of Bambusa and the paraphyletic origin of the genus Dendrocalamus. |