IN VITRO SEED GERMINATION OF DECALEPIS HAMILTONII AN ENDANGERED MEDICINAL PLANT USING GIBBERELLIC ACID AND COCONUT WATEREitisha Sandecha, Dipesh Chikane, Suresh Jagtap, Arvind Kadus and Neelambika MetiAbstract A highly efficient protocol was developed for germination of seedlings from mature seed pods of Decalepis hamiltonii Wight. and Arn. It was found that gibberellic acid (GA3) had a synergistic effect on germination in combination with coconut water. This protocol uses gibberellic acid for germination of seeds, which was more in the presence of coconut water. The maximum number of seedlings per culture with root formation was observed on the medium containing gibberellic acid (0.5 mg/l) full strength media with coconut water (10 %) showed 40% germination, while gibberellic acid (1.5 mg/l) half strength media with coconut water (5 %) resulted in to vigorous and synchronous growth of seedlings up to 50% respectively in comparison with control. The seedlings were kept for acclimatization, and their field survival rate was 50-75 %. |