Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 8, Issue 3, 2006; Page No.(651-654)

MOLECULAR CHARACTERIZATION OF CHROMIUM RESISTANT BACTERIA FROM CHROMIUM CONTAMINATED TANNERY EFFLUENTS

G. VIJAIYANSIVA, K. MOHAMMED HUSSAIN AND .J.PRASANNA LAKSHMI

Abstract

A gram negative bacterium (Salmonella) was isolated from a chromium-contaminated site that was capable of inducing a plasmid when grown in chromium enriched medium. The isolate was able to induce a high molecular weight fragment from its genomic DNA when it was incubated with the high concentration of 2mMollit-1 Chromium. Analysis of the genomic DNA and plasmid DNA from the isolate revealed a high molecular weight genomic DNA breakups and two lower molecular weight plasmid DNA, Hence the mechanism of resistance in Salmonella is plasmid mediated from its own genomic DNA breakup.

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