Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 9, Issue 3, 2007; Page No.(683 -690)

UNSTRUCTURED KINETIC MODELS FOR LIPASE PRODUCTION BY BACILLUS SPHAERICUS

ARAVINDAN RAJENDRAN, ASHOK BALAJI SUNDARAMURTHY AND VIRUTHAGIRI THANGAVELU

Abstract

Unstructured kinetic modeling for cell growth, lipase production and substrate utilization are presented for lipase production by Bacillus sphaericus in batch submerged fermentation. The unstructured models, Logistic model for cell growth, Monod incorporated Luedeking- Piret model for lipase production and Modified Logistic model incorporated modified Luedeking-Piret model for substrate utilization where capable of predicting with high coefficient of determination (R2) values of 0.9977, 0.9871 and 0.9966 respectively. The predicted profile of the fermentation are in good agreement with the experimental profile, indicated that the unstructured models were able to describe the fermentation profile effectively. Estimated parameters of the Luedeking- Piret model for lipase production indicate that the production of lipase by Bacillus sphaericus is growth-associated.

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