Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 5 Issue 2, 2003; Page No.(193-196 )

STRATEGIC PRIMING IN FISH VACCINE DEVELOPMENT

SUDHIR U. MESHRAM, A.S. SHANWARE AND G.B. SHINDE

Abstract

Vaccination is one of the methods of lowering risks associated with the occurrence of diseases at fry & fingerlings stages. Specificity, immunogenic memory, safety & potency are the key elements exploited by vaccination. An interdependence of many factors such as antigen dose, nature of the antigen, route of administration and adjuvants may affect the out come of the immune response of a test vaccine. Immunization of fish stocks by injection is economically as well as practically prohibitive and hence alternatively, efficient method of immunization is being sought in the present investigation. The extensive field survey of Aquabodies in Vidarbha region revealed the ubiquitous nature of Pseudomonas sp. with a variety of bacterial diseases, made it a principal organism to process for vaccine preparation method involving a series of critical sterility tests. The studies revealed that the method provided good protection because of stimulation of integumentary immunity against experimental challenge. It is extremely difficult to assess these tests successfully at laboratory conditions for the challenge may not be the same for all groups, the minimum mortality rate may not be achieved in control and intercurrent diseases may account for more than 10% of the mortality.

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