PHYSICOCHEMICAL AND MICROBIAL ANALYSIS OF SOIL AND WATER SAMPLES OBTAINED FROM NOYYAL RIVER BASIN; BACTERIAL DYE DECOLORIZATION STUDIESP. KANMANI, K. KUMARESAN*, J. ARAVIND, P. ARUN SATHYASEELAN AND K. SIBIAbstract An analysis of physico-chemical and bacteriological characteristics of soil and water samples with varying levels of exposure to textile industrial effluents, obtained from the Noyyal river basin, was performed in this study. The results shed some light on the extent of pollution in the area and its implications. pH, electrical conductivity, available N, P, and K, were the soil parameters analyzed. pH, Turbidity, Chlorides, TSS and TDS were the parameters investigated for water samples. Microbial screening was also performed and viable counts of halophilic bacteria were obtained by serial dilution and plating of the samples in a growth medium containing 23% (v/v) NaCl. Six bacterial colonies in the plates exhibiting visible difference in morphology were subcultured and maintained as pure cultures on agar slants. They were employed for biodegradation of three synthetic azo dyes, namely, Reactive Red, Reactive Blue, Reactive Yellow and an effluent dye. The isolate KAS1 imparted a maximum of 90% decolorization of Reactive Red and 57% decolorization of Reactive Blue. 61% decolorization of Reactive Yellow was brought about by the strain PAS1 and 75% decolorization of effluent dye by PAS2. Two of the isolates KAS1 and PAS2 were identified by Matrix Assisted Light Desorption/Ionization Time of Flight Mass Spectrometry (MALDI-TOF MS) as belonging to Alcaligenes sp and Bacillus sp, respectively.
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