Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 18, Issue 4, 2016; Page No.(861-868)

IDENTIFICATION OF DIFFERENTIALLY EXPRESSED GENES INDUCED BY POLY ETHYLENE GLYCOL IN MAIZE (ZEA MAYS L.) CALLUS QUALITY IMPROVEMENT

MANAL M. ABDEL-RAHMAN, ASMAA M. EL-FARAMAWY AND IBRAHIM E. MOUSA

Abstract

Maize (Zea mays L.) callus acts as the most suitable materials for genetic transformation that is used in molecular genetic research. In the present study, the quality improvement of non-embryogenic (NE) to embryogenic calli (E) is very important. The transcript abundances of associated gene were investigated during callus treatment with 10% and 20% polyethylene glycol (PEG). Differential display technique was used to study the modification of maize HiII genotype calli. By applying seven arbitrary primers, mRNAs were used to study the difference between NE and E calli as a result of PEG treatment. Results indicated that significant variations were shown among the treated samples examined with primers A1, R14, P2 and A13. Six different down regulated genes were observed, selected genes were amplified in callus samples with primer A1, R14, and p2, respectively. 30 up regulated genes were observed in treated samples comparison to control. Nine of these genes were eluted, purified and sequenced. These genes might participate in the transformation of NE to E callus. Also, results revealed that their deduced amino acids, ATFP protein of Zea mays, putative pol protein, Opie2b gag protein, putative gag/pol protein, amino transferees class III and Putative gag/pol precursor (Zea mays) protein were expressed as a result of PEG treatment. These results suggested that PEG as water regulator may have contributed to change NE calli to E calli by enhancing protein metabolism and its related genes.

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