Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 18, Issue 4, 2016; Page No.(967-973)

DIVERSITY OF CELLULOSE DEGRADING ACTINOMYCETES IN WASTE LANDFILL ECOSYSTEM

SUBATHRA LAVAN, M. SURESH KUMAR, D. KAVITHA and S. ILAKKIYA

Abstract

The microbial diversity of cellulolytic actinomycetes was experimented in a waste landfill soil in Salem district, Tamilnadu, India. In this study, among 33 isolates, 11 Actinomycetes strains showed good Cellulase enzyme activity via plate-clearing assays. Quantification of cellulase enzyme activity in these 11 strains demonstrated ACT-1, ACT-2 and ACT-3 as efficient producers of cellulase enzyme (70µg/mL, 65 µg/mL and 58 µg/mL). The optimum pH and temperature for cellulase enzyme activity was determined to be pH 6 for ACT-1 and 2, pH 7.0 for ACT-3 and 50 oC was found to be the optimum temperature for all threestrains. Sequence identification using NCBI-BLAST revealed ACT-1 and ACT-2 belonging to Streptomyces albogriseolusand ACT-3 was assigned to Streptomyces griseoflavusstrain and their respective sequences were deposited in the Genbank database of NCBI with accession numbers of KU341336 and KU720585, respectively. The neighbour-joining phylogenetic tree showed higher evolutionary relationship with Streptomyces albogriseolus followed by Streptomyces Griseoflavus.

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