Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 19, Issue 2, 2017; Page No.(331-336)

TANNIN EXTRACT CHARACTERIZATION OF YOUNG MANGROVE RHIZOPHORA MUCRONATA LEAVES AS INGREDIENTS FOR DIABETIC FUNCTIONAL FOOD

HARDOK, BAMBANG BUDI SASMITO1 AND YUNITA EKA PUSPITASARI

Abstract

Young leaves are often used as food and traditional medicines. Likewise the Rhyzophora plant that is widely used as a medicine and food are part of a young plant. The purpose of this study was to characterize the in vitro activity of antidiabetic using α-glucosidase enzymes and antioxidant activity with DPPH of young leaves tannin extract R. mucronata. Tannin extraction methods was carried out using acetone-water 70-30 and sonication at 40Hz amplitude for 30 minutes. Condensed tannins fractionation process is done by separating funnel using a solvent, hexane, chloroform, and ethyl acetate. The results showed that the inhibitory activity of the enzyme α-glucosidase by condensed tannins fraction is higher than the crude extract tannin young leaves R. mucronata and acarbose, as indicated by the IC50 value were 25.08 ± 0.88 μg/mL (crude extract tannins) and 8.07 ± 0.58 μg/mL (fraction of condensed tannins), and 10.60 ± 0.20 μg mL (acarbose), respectively. Condensed tannins fractionation process reduces levels of total phenolic, total tannins and total flavonoids, but increases levels of condensed tannins. Although antidiabetic in vitro activity of the crude extract tannins and condensed tannins fraction is high, but the antioxidant activity were very weak as demonstrated IC50 value were 981494.70 ± 255.76 μg/mL (crude extract tannins) and 1725.18 ± 25.97 μg/ mL (fraction of condensed tannins).

Enter your contact information below to receive full paper.
Your Name :
Email:
Phone:
City:
Cost of Full Paper: Rs.75 for Indian Nationals or $20 (USD) for international subscribers.
By clicking on Request Paper you Agree to pay the above mentioned cost per paper.
  
Journal Issues
Vol 19, Issue 2, 2017
Vol 19, Issue 1, 2017
Vol 18, Issue 4, 2016
Vol 18, Issue 3, 2016
Vol 18, Issue 2, 2016
Vol 18, Issue 1, 2016
Vol 17, Issue 4, 2015
Vol 17, Issue 3, 2015
Vol. 17 Special Issue 2015
Vol 17, Issue 2, 2015
Vol 17, Issue 1, 2015
Vol 16, Issue 4, 2014
Vol 16, Issue 3, 2014
Vol 16, Issue 2, 2014
Vol 16, Issue 1, 2014
Vol 15, Issue 4, 2013
Vol 15, Issue 3, 2013
Vol 15, Issue 2, 2013
Vol 15 Issue 1, 2013
Vol 14, Issue 4, 2012
Vol 14, Issue 3, 2012
Vol 14, Issue 2, 2012
Vol.14, Issue 1, 2012
Vol.13, Issue 4, 2011
Vol.13, Issue 3, 2011
Vol.13, Issue 2, 2011
Vol.13, Issue 1, 2011
Vol.12, Issue 4, 2010
Vol.12, Issue 3, 2010
Vol.12, Issue 2, 2010
Vol.12, Issue 1, 2010
Vol 11, Issue 4, 2009
Vol 11, Issue 3, 2009
Vol 11, Issue 2, 2009
Vol 11, Issue 1, 2009
Vol 10, Issue 4, 2008
Vol 10, Issue 3, 2008
Vol 10, Issue 3, 2008
Vol 10, Issue 2, 2008
Vol 10, Issue 1, 2008
Vol 9, Issue 4, 2007
Vol 9, Issue 3, 2007
Vol 9, Issue 2, 2007
Vol 9, Issue 1, 2007
Vol 8, Issue 4, 2006
Vol 8, Issue 3, 2006
Vol 08, Issue 2, 2006
Vol 08, Issue 1, 2006
Vol 7 Issue 4, 2005
Vol 07, Issue 3, 2005
Vol 07, Issue 2, 2005
Vol 6 Issue 4, 2004
Vol 6 Issue 3, 2004
Vol 6 Issue 2, 2004
Vol 6 Issue 1, 2004
Vol 5 Issue 3, 2003
Vol 5 Issue 2, 2003
Vol 04, Issue 4, 2002
Vol 04, Issue 1, 2002
Vol 3 Issue 1-2, 2001
Vol 1 Issue 1-2, 2000
Vol 1 Issue 3-4, 1999
Vol 1 Issue 1-2, 1999
Looking for Past Issues? Click here to get them!!