Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 19, Issue 2, 2017; Page No.(419-424)

INFLUENCE OF FUNGAL DELIGNIFICATION ENZYMES IN TREATED COIR PITH FOR POLLUTION MANAGEMENT

PRIYA V., SAMPATH KUMAR M.C. AND N. BALASUBRAMANYA

Abstract

Lignin is one of the major classes of any structural material that aids in the support of tissues. High lignin content in the coir pith make its natural degradation much slower due to the lignincellulose complex. It is opportune to know that the engineered microbes act as ligninolytic fungi and helps in the degradation of lignin by the release of the enzymes. These enzymes are responsible for the degradation of recalcitrant tissues in the lignin, thus tackle the problem of bioconversion of substrate, coir pith. This study helps in the detection of major enzymes as a result of degradation of lignin, ending in the usage of risk free, pollution free coir pith. This study is significant as the by-product coir pith is being utilized for production of commercially valuable enzymes today whose non-degraded form have serious effects when used commercially. In this study, ligninolytic microbes such as Phanerochaete chrysosporium and Trichoderma viride were trialed for the experimentation of lignin degradation in the treated coir pith with Rock phosphate and Clostridium perfringens for the reduction of electrical conductivity. On the whole, the modest results of enzyme detection such as cellulases, laccases, lignin peroxidases, proteases, xylanases were obtained in both the fungal organisms in treated coir pith; This proved the success of the lignin degradation in an economic, risk free and environmental friendly method.

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