STRUCTURE-FUNCTION ANALYSIS OF THE MULTI-DOMAIN BETA-KETOACYL SYNTHASE INVOLVED IN THE PRODUCTION OF EICOSAPENTAENOIC ACID, DOCOSAHEXAENOIC ACID AND ANTIBIOTICS BY IN-SILICO COMPARATIVE APPROACHES AND PHYLOGENETIC ANALYSISALI BIN THANI, SALWA AL-THAWADI AND AFNAN FREIJEAbstract Production of polyunsaturated fatty acids such as eicosapentaenoic (EPA) and docosahexaenoic (DHA) by psychro tolerant piezophilic bacterial species requires the involvement of polyketide synthase (PKS-like) enzymes. These enzymes are also involved in the production of antibiotics by different bacterial species. The purpose of the current in-silico analysis is to infer the phylogenetic relationship between strains capable of producing ω3 fatty acids such (e.g., EPA, DHA) and compare them to the Polyketide synthases involved in the production of antibiotics. The Chain Length Factor (CLF) is used to facilitate and examine the differences between the three different groups of Polyketide synthases. The current work has pin point the evolution among different sites in CLF proteins. Maximum-Likelihood analysis elucidated that most of these sites were present in the loops owing to structure variability in the examined proteins and differences that might be related to folding stability for proper attachment when the active site of the protein interacts with its ligand.
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