Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 20, Issue 2, 2018; Page No.(685-688)

ISOLATION AND IDENTIFICATION OF HYDROCARBON DEGRADING BACTERIA FROM PETROLEUM CONTAMINATED SOIL OF ASSAM

ARCHANA KALITA , MANAB DEKA , ANIRUDDHA SARMA AND DEEPALI DEKA

Abstract

In the present research, attempts were made to isolate hydrocarbon degrading bacteria using enriched media with crude oil and diesel as sole carbon source. Among 10 different strains, two strains namely Strain 5 and 6 showed remarkable growth in crude oil and diesel and a high OD value (> 0.2) at 600 nm which was used as a preliminary screening for bioremediation. Based on morphology, physiology and biochemical analysis the two strains were identified as Gordonia sp. and Pseudomonas sp. Later 16s molecular characterization of the two strains were carried out and the data were submitted to the genetic sequence database at the National Center for Biotechnology Information (NCBI). The GenBank ID (http://www.ncbi.nlm.nih.gov) of these strains were LC279537 and LC279538. They were molecularly identified as Gordonia amicalis and Pseudomonas putida.

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